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Molecular Biology
A Guide to the NGS Workflow
NGS Library Preparation and Sample Prep
KAPA Pure Beads
KAPA Pure Beads from Roche
Be the first to write a review!
Citations:
(236)
Supplier Page
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Roche for
KAPA Pure Beads
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Product Specs
Item
KAPA Pure Beads
Company
Roche
Price
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Catalog Number
07983280001
Quantity
30 mL
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (236)
(101)
Dynamics of SARS-CoV-2 Alpha (B.1.1.7) variant spread: The wastewater surveillance approach
Environmental Research
May 15, 2022
Albert Carcereny, David Garcia-Pedemonte, Adán MartÃnez-Velázquez, Josep Quer, Damir Garcia-Cehic, Josep Gregori, Andrés Antón, Cristina Andrés, Tomà s Pumarola, Carme Chacón-Villanueva,
et al.
amplicons in two separate pools) encompassing 40 cycles of
amplification
, and
DNA purification
using the
Kapa Pure Beads
(
Roche
), (iii) quantitation using the
Qubit® dsDNA HS Assay Kit
(
Thermo Fisher Scientific
, Waltham,
(102)
Vaccine breakthrough infections with SARS-CoV-2 Alpha mirror mutations in Delta Plus, Iota, and Omicron
Journal of Clinical Investigation
May 2, 2022
Brenda MartÃnez-González, LucÃa Vázquez-Sirvent, MarÃa E. Soria, Pablo MÃnguez, Llanos Salar-Vidal, Carlos GarcÃa-Crespo, Isabel Gallego, Ana I. de Ãvila, Carlos Llorens, Beatriz Soriano,
et al.
patients. To obtain DNA pools,
PCR
products were adjusted to 4 × 109 molecules/μL and were purified using
Kapa Pure Beads
(Kapabiosystems,
Roche
). Pools quantifications were performed using Qubit as previously described and
(103)
SARS-CoV-2 Point Mutation and Deletion Spectra and Their Association with Different Disease Outcomes
Microbiology Spectrum
April 27, 2022
Brenda MartÃnez-González, MarÃa Eugenia Soria, LucÃa Vázquez-Sirvent, Cristina Ferrer-Orta, Rebeca Lobo-Vega, Pablo MÃnguez, Lorena de la Fuente, Carlos Llorens, Beatriz Soriano, Ricardo Ramos,
et al.
adjusted
PCR
products to 4 × 109 molecules/μL before generating DNA pools that were purified using
Kapa pure beads
(Kapabiosystems,
Roche
), quantified them using Qubit as described previously (38–40), and then fixed
(104)
Optimizing bulk segregant analysis of drug resistance using Plasmodium falciparum genetic crosses conducted in humanized mice
iScience
April 15, 2022
Katelyn Vendrely Brenneman, Xue Li, Sudhir Kumar, Elizabeth Delgado, Lisa A. Checkley, Douglas A. Shoue, Ann Reyes, Biley A. Abatiyow, Meseret T. Haile, Rupam Tripura,
et al.
KAPA HyperPlus Kit
,
Roche
,Lot 0000123512 NEXTflex Dual-Indexed DNA Barcodes (1-96),PerkinElmer,Cat# NOVA-514160
KAPA Pure Beads
,
Roche
,Cat# 0798328001
Quant-iT PicoGreen dsDNA Assay Kit
,
Invitrogen
,Cat#
Q33120
KAPA Library Quant
(105)
FKBP‐type peptidyl‐prolyl
cis‐trans
isomerase interacts with the movement protein of tomato leaf curl New Delhi virus and impacts viral replication in
Nicotiana benthamiana
Molecular Plant Pathology
April 1, 2022
Ho‐Hsiung Chang, Chia‐Hwa Lee, Chung‐Jan Chang, Fuh‐Jyh Jan
Double‐Strand cDNA Synthesis Kit (Takara) following the manufacturer's recommendations. DNA was cleaned up with
KAPA Pure Beads
(KAPABiosystem;
Roche
) followed by end repair and A‐tailing reaction with the
KAPA HyperPrep Kit
(KAPABiosystems).
(106)
Smallpox vaccination induces a substantial increase in commensal skin bacteria that promote pathology and influence the host response
PLoS pathogens
April 1, 2022
Shmeleva EV, Gomez de Agüero M, Wagner J, Enright AJ, Macpherson AJ, Ferguson BJ, Smith GL.
amplification
with 11 and 22
PCR
cycles for the first and second rounds of amplifications, respectively.
Kapa Pure beads
(KappaBiosystems, Cat # KK8002,
Roche
) were applied
for purification
and size selection of DNA with
(107)
Large-scale fungal strain sequencing unravels the molecular diversity in mating loci maintained by long-term balancing selection
PLOS Genetics
March 31, 2022
David Peris, Dabao Sun Lu, Vilde Bruhn Kinneberg, Ine-Susanne Methlie, Malin Stapnes Dahl, Timothy Y. James, Håvard Kauserud, Inger Skrede
gDNA samples were cleaned on a small volume
Mosquito
liquid handler (
TTP labtech
) with a
1:1
ratio of
Kapa Pure beads
(
Roche
, Basel, Switzerland) and eluted in Tris-Cl, pH 8.0. Library preparation was carried out with
(108)
First report on metabarcoding analysis of gut microbiome in Island Flying Fox (Pteropus hypomelanus) in island populations of Malaysia
Biodiversity Data Journal
March 22, 2022
Nur Syafika Mohd-Yusof, Muhammad Abu Bakar Abdul-Latiff, Abd Rahman Mohd-Ridwan, Aqilah Sakinah Badrulisham, Nursyuhada Othman, Salmah Yaakop, Shukor Md-Nor, Badrul Munir Md-Zain
with a final elongation at 72°C for 5 min. The
PCR
products were purified using 0.7x volume ratio of
Kapa pure beads
(
KAPA Biosystems
, USA). Then, the second
PCR
was conducted for indexing amplicons from the first
PCR
(109)
Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
Nucleic Acids Research
March 21, 2022
Keita Okada, Kanae Aoki, Teruyuki Tabei, Kota Sugio, Katsunori Imai, Yuki Bonkohara, Yusuke Kamachi
agarose gel electrophoresis
,
PCR
products were purified using
NucleoSpin Gel and PCR Clean-up
(
Macherey-Nagel
) or
KAPA Pure Beads
(
Kapa Biosystems
). Purified
PCR
products were Sanger sequenced using primers listed in Supplementary
(110)
Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L
Viruses
February 24, 2022
Reimar Johne, Katja Schilling-Loeffler, Rainer G. Ulrich, Simon H. Tausch
HotStart Ready Mix 2× (
Roche Diagnostics
) after
ligation
. The resulting library was purified using
KAPA Pure Beads
(
Roche Diagnostics
) and size distribution monitored on the Fragment Analyzer 5200 (Agilent Technologies,
(111)
A Parallel Tracking of Salivary and Gut Microbiota Profiles Can Reveal Maturation and Interplay of Early Life Microbial Communities in Healthy Infants
Microorganisms
February 18, 2022
Sofia Reddel, Giuseppe Rubens Pascucci, Silvia Foligno, Federica Del Chierico, Pamela Vernocchi, Alessandra Marzullo, Maria Grazia Pattumelli, Paolo Palma, Guglielmo Salvatori, Lorenza Putignani
for 30 s, and final extension step at 72 °C for 5 min. The DNA amplicons were purified with 20 µL of
KAPA Pure Beads
(
Roche Diagnostics
, Germany). The second
PCR
step was performed by Nextera indexes (Illumina, USA) to
(112)
Longitudinal Monitoring of DNA Viral Loads in Transplant Patients Using Quantitative Metagenomic Next-Generation Sequencing
Pathogens
February 11, 2022
Ellen C. Carbo, Anne Russcher, Margriet E. M. Kraakman, Caroline S. de Brouwer, Igor A. Sidorov, Mariet C. W. Feltkamp, Aloys C. M. Kroes, Eric C. J. Claas, Jutte J. C. de Vries
were ligated using unique dual-index adapters (ArcBio) at 20 °C for 15 min and purified using magnetic
Kapa Pure Beads
(
Roche
, Basel, Switzerland). No
RNase
treatment was included in the
procedure
, and
human
DNA was depleted
(113)
Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5
hi
PD-1
hi
T follicular helper cells
Immunity
February 8, 2022
Yeh CH, Finney J, Okada T, Kurosaki T, Kelsoe G.
Biotechnologies,https://www.immunoseq.com/
assays
/
KAPA HyperPlus Kit
,
Roche
Molecular Systems,Cat#
07962401001
KAPA Pure Beads
,
Roche
Molecular Systems,Cat#07983280001 LIVE/DEAD™ Fixable Near-IR Dead
Cell Stain
Kit,
Thermo Fisher Scientific
(114)
Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice
Molecular medicine reports
February 1, 2022
Yeh CC, Liu HM, Lee MC, Leu YL, Chiang WH, Chang HH, Lee TY.
order to select cDNA fragments of ~150 bp in length, the library fragments were purified using the
KAPA Pure Beads
System (cat. no.
07983298001
;
KAPA Biosystems
; Roche Diagnostics GmbH). cDNA was synthesized using a
(115)
Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2
+
DRG sensory neurons
Nature neuroscience
February 1, 2022
Yang NJ, Isensee J, Neel DV, Quadros AU, Zhang HB, Lauzadis J, Liu SM, Shiers S, Belu A, Palan S,
et al
.
subsequently as part of the same workflow. Following
amplification
, residual primers were eluted away using
KAPA Pure Beads
in a 0.63× SPRI-based cleanup.
qPCR
with the
KAPA
Library Quantification kit was run to confirm the
(116)
The Roles of microRNA-Long Non-coding RNA-mRNA Networks in the Regulation of Leaf and Flower Development in Liriodendron chinense
Frontiers in Plant Science
January 27, 2022
Zhonghua Tu, Hui Xia, Lichun Yang, Xinyu Zhai, Yufang Shen, Huogen Li
synthesized by the
First-Strand cDNA Synthesis Kit
(NEB, United States) and then amplified by
PCR
.
KAPA Pure Beads
(
Roche
, United States) were used to select fragments of a specific length. The selected fragments were
(117)
Detection of Low-Copy Human Virus DNA upon Prolonged Formalin Fixation
Viruses
January 12, 2022
Outi I. Mielonen, Diogo Pratas, Klaus Hedman, Antti Sajantila, Maria F. Perdomo
enrichment, the libraries were amplified 3 × 13-25 cycles. The cleanup steps were performed with 0.8×
KAPA Pure Beads
(
Roche
, Basel, Switzerland). The enriched libraries were quantified with KAPA Library Quantification
(118)
Persistence of SARS CoV-2 S1 Protein in CD16+ Monocytes in Post-Acute Sequelae of COVID-19 (PASC) up to 15 Months Post-Infection
Frontiers in Immunology
January 10, 2022
Bruce K. Patterson et al.
amplification
and libraries were cleaned up using 0.5X right sided cleanup and 1.2X left sided cleanup using
Kapa Pure Beads
(
Roche
Catalog no l7983298001). Final libraries were quantified using Ion Library TaqMan Quantitation
(119)
Absence of Bacteria Permits Fungal Gut-To-Brain Translocation and Invasion in Germfree Mice but Ageing Alone Does Not Drive Pathobiont Expansion in Conventionally Raised Mice
Frontiers in aging neuroscience
January 1, 2022
Parker A, James SA, Purse C, Brion A, Goldson A, Telatin A, Baker D, Carding SR.
C. albicans DNA) were included in each
PCR
run. Following ITS1
PCR
, a 0.7x SPRI
purification
using
KAPA Pure Beads
(
Roche
, Wilmington, MA, United States) was performed and the purified DNA was eluted in 20 μl of 10
(120)
Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo
Oxidative medicine and cellular longevity
January 1, 2022
Liu WC, Chuang HC, Chou CL, Lee YH, Chiu YJ, Wang YL, Chiu HW.
fragments with a length of 300~400 bp were selected, and library fragments were extracted with the
KAPA Pure Beads
system (
KAPA Biosystems
, Roche, Basel, Switzerland). The library was increased with
KAPA HiFi HotStart ReadyMix
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KAPA Pure Beads from Roche
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